Targeted Genome Editing for Crop Improvement
نویسندگان
چکیده
منابع مشابه
Genome Editing With Targeted Deaminases
Luhan Yang, Adrian W. Briggs*, Wei Leong Chew*, Prashant Mali, Marc Guell, John Aach, Daniel Bryan Goodman, David Cox, Yinan Kan, Emal Lesha Venkataramanan Soundararajan, Feng Zhang, George Church 1 Department of Genetics, Harvard Medical School, Boston, MA 2 Program in Biological and Biomedical Sciences, Harvard Medical School, Boston, MA 3 Harvard-MIT Division of Health Science and Technology...
متن کاملGene Editing and Crop Improvement Using CRISPR-Cas9 System
Advancements in Genome editing technologies have revolutionized the fields of functional genomics and crop improvement. CRISPR/Cas9 (clustered regularly interspaced short palindromic repeat)-Cas9 is a multipurpose technology for genetic engineering that relies on the complementarity of the guideRNA (gRNA) to a specific sequence and the Cas9 endonuclease activity. It has broadened the agricultur...
متن کاملCas9-based tools for targeted genome editing and transcriptional control.
Development of tools for targeted genome editing and regulation of gene expression has significantly expanded our ability to elucidate the mechanisms of interesting biological phenomena and to engineer desirable biological systems. Recent rapid progress in the study of a clustered, regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated (Cas) protein system in bacteria has fac...
متن کاملAdvancing Crop Transformation in the Era of Genome Editing.
Plant transformation has enabled fundamental insights into plant biology and revolutionized commercial agriculture. Unfortunately, for most crops, transformation and regeneration remain arduous even after more than 30 years of technological advances. Genome editing provides novel opportunities to enhance crop productivity but relies on genetic transformation and plant regeneration, which are bo...
متن کاملTargeted genome editing across species using ZFNs and TALENs.
Evolutionary studies necessary to dissect diverse biological processes have been limited by the lack of reverse genetic approaches in most organisms with sequenced genomes. We established a broadly applicable strategy using zinc finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs) for targeted disruption of endogenous genes and cis-acting regulatory elements in d...
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ژورنال
عنوان ژورنال: Plant Breeding and Biotechnology
سال: 2015
ISSN: 2287-9366
DOI: 10.9787/pbb.2015.3.4.283